SrasV1, Main, Exploration, bibRecord, 001056

Crystal Structure of Feline Infectious Peritonitis Virus Main Protease in Complex with Synergetic Dual Inhibitors.

Identifieur interne : 001056 ( Main/Exploration ); précédent : 001055; suivant : 001057

Crystal Structure of Feline Infectious Peritonitis Virus Main Protease in Complex with Synergetic Dual Inhibitors.

Auteurs : Fenghua Wang [République populaire de Chine] ; Cheng Chen [République populaire de Chine] ; Xuemeng Liu [République populaire de Chine] ; Kailin Yang [États-Unis] ; Xiaoling Xu [République populaire de Chine] ; Haitao Yang [République populaire de Chine]

Source :

Journal of virology [ 1098-5514 ] ; 2016.

RBID : pubmed:26656689

Descripteurs français

English descriptors

KwdEn :
- Amino Acid Sequence, Coronavirus, Feline (enzymology), Crystallography, X-Ray, Cysteine Endopeptidases (chemistry), Cysteine Endopeptidases (metabolism), Models, Molecular, Molecular Sequence Data, Protease Inhibitors (chemistry), Protease Inhibitors (metabolism), Protein Binding, Protein Conformation, Zinc (chemistry), Zinc (metabolism).
MESH :
- chemical , chemistry : Cysteine Endopeptidases, Protease Inhibitors, Zinc.
- enzymology : Coronavirus, Feline.
- chemical , metabolism : Cysteine Endopeptidases, Protease Inhibitors, Zinc.
- Amino Acid Sequence, Crystallography, X-Ray, Models, Molecular, Molecular Sequence Data, Protein Binding, Protein Conformation.

Abstract

Coronaviruses (CoVs) can cause highly prevalent diseases in humans and animals. Feline infectious peritonitis virus (FIPV) belongs to the genus Alphacoronavirus, resulting in a lethal systemic granulomatous disease called feline infectious peritonitis (FIP), which is one of the most important fatal infectious diseases of cats worldwide. No specific vaccines or drugs have been approved to treat FIP. CoV main proteases (M(pro)s) play a pivotal role in viral transcription and replication, making them an ideal target for drug development. Here, we report the crystal structure of FIPV M(pro) in complex with dual inhibitors, a zinc ion and a Michael acceptor. The complex structure elaborates a unique mechanism of two distinct inhibitors synergizing to inactivate the protease, providing a structural basis to design novel antivirals and suggesting the potential to take advantage of zinc as an adjunct therapy against CoV-associated diseases.

DOI: 10.1128/JVI.02685-15
PubMed: 26656689

Affiliations:

Le document en format XML

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<term>Crystallography, X-Ray</term>
<term>Cysteine Endopeptidases (chemistry)</term>
<term>Cysteine Endopeptidases (metabolism)</term>
<term>Models, Molecular</term>
<term>Molecular Sequence Data</term>
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<term>Inhibiteurs de protéases ()</term>
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<term>Séquence d'acides aminés</term>
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<term>Protease Inhibitors</term>
<term>Zinc</term>
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<term>Inhibiteurs de protéases</term>
<term>Zinc</term>
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<term>Crystallography, X-Ray</term>
<term>Models, Molecular</term>
<term>Molecular Sequence Data</term>
<term>Protein Binding</term>
<term>Protein Conformation</term>
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<term>Cristallographie aux rayons X</term>
<term>Cysteine endopeptidases</term>
<term>Données de séquences moléculaires</term>
<term>Inhibiteurs de protéases</term>
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<front><div type="abstract" xml:lang="en">Coronaviruses (CoVs) can cause highly prevalent diseases in humans and animals. Feline infectious peritonitis virus (FIPV) belongs to the genus Alphacoronavirus, resulting in a lethal systemic granulomatous disease called feline infectious peritonitis (FIP), which is one of the most important fatal infectious diseases of cats worldwide. No specific vaccines or drugs have been approved to treat FIP. CoV main proteases (M(pro)s) play a pivotal role in viral transcription and replication, making them an ideal target for drug development. Here, we report the crystal structure of FIPV M(pro) in complex with dual inhibitors, a zinc ion and a Michael acceptor. The complex structure elaborates a unique mechanism of two distinct inhibitors synergizing to inactivate the protease, providing a structural basis to design novel antivirals and suggesting the potential to take advantage of zinc as an adjunct therapy against CoV-associated diseases.</div>
</front>
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Crystal Structure of Feline Infectious Peritonitis Virus Main Protease in Complex with Synergetic Dual Inhibitors.

Crystal Structure of Feline Infectious Peritonitis Virus Main Protease in Complex with Synergetic Dual Inhibitors.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

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Pour générer des pages wiki

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